Dept of Ob/Gyn and Reproductive Sciences
Glycobiology Research and Training Center
UC San Diego
Warren G Lewis, Ph.D.
Selected Publications
Biochemical, molecular, and microbial studies of bacterial vaginosis
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Lewis, W.G.; Robinson, L.S.; Perry, J.C.; Bick, J.L.; Peipert, J.F.; Allsworth, J.E.; Lewis, A.L. Hydrolysis of the secreted sialoglycoprotein Immunoglobulin A (IgA) in ex vivo and biochemical models of bacterial vaginosis. J. Biol. Chem. 2012; 287(3): 2079-89. PMID: 22134918.
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Lewis, W.G.; Robinson, L.S.; Gilbert, N.M.; Perry, J.C.; Lewis, A.L. Degradation, foraging and depletion of mucus sialoglycans by the vagina-adapted Actinobacterium Gardnerella vaginalis. J. Biol. Chem. 2013; 288(17), 12067-79. PMID: 23479734.
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Robinson L.S., Schwebke J., Lewis W.G., and Lewis A.L. Identification and characterization of NanH2 and NanH3, the enzymes responsible for sialidase activity in Gardnerella vaginalis. 2019 J Biol Chem pii: jbc.RA118.006221. doi: 10.1074/jbc.RA118.006221. PMID: 30723162.
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Agarwal KA, Robinson LS, Aggarwal S, Lynne Foster LR, Hernandez-Leyva A, Lin H, Tortelli BA, O’Brien VP, Miller L, Kau AL, Reno H, Gilbert NM, Lewis WG*, Lewis AL*. Glycan cross-feeding supports mutualism between Fusobacterium and the vaginal microbiota. PLoS Biology, 2020 Aug 25;18(8):e3000788.doi: 10.1371/journal.pbio.3000788. eCollection 2020 Aug. PMID: 32841232 PMCID: PMC7447053 * Co-Corresponding authors
Degradation of protein and carbohydrate components of mucus related to microbial interactions with the gastrointestinal tract.
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Robinson L.S., Lewis W.G., Lewis A.L. The Sialylate O-acetylesterase EstA from Gut Bacteroides Species Enables Sialidase-Mediated Cross-species Foraging of 9-O-acetylated Sialoglycans. J Biol Chem. 2017 pii: jbc.M116.769232. doi: 10.1074/jbc.M116.769232. PMID: 28526748.
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Luo Q., Kumar P., Vickers T.J., Sheikh A., Lewis W.G., Rasko D.A., Sistrunk J., Fleckenstein J.M. Enterotoxigenic Escherichia coli secretes a highly conserved mucin-degrading metalloprotease to effectively engage intestinal epithelial cells. Infect Immun. 2014 Feb;82(2):509-21. doi: 10.1128/IAI.01106-13. PubMed PMID: 24478067
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Kumar P., Luo Q., Vickers T.J., Sheikh A., Lewis W.G., Fleckenstein J.M. EatA, an immunogenic protective antigen of enterotoxigenic Escherichia coli, degrades intestinal mucin. Infect Immun. 2014; 82(2):500-8. doi: 10.1128/IAI.01078-13. PMID: 24478066
Glycan biosynthesis & its functions in pathogens.
To understand how bacteria make surface carbohydrates, and the functional consequences of the saccharide coating of pathogen cell surfaces, I apply biochemical and cellular assays to understand the origins & biological consequences of glycan decoration in bacteria, nematodes and other parasites.
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Cai CW, O’Shea A, Eickhoff CS, Guo H, Lewis WG, Beverley SM and Hoft DF. Use of Leishmania major parasites expressing a recombinant Trypanosoma cruzi antigen as live vaccines against Chagas disease. Front. Microbiol. 13:1059115 2022. doi: 10.3389/fmicb.2022.1059115
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Lubin, J.B.; Lewis, W.G.; Gilbert, N.; Weimer, C.M.; Almagro-Moreno, S.; Boyd, E.F.; Lewis, A.L. Host-like carbohydrates promote bloodstream survival of Vibrio vulnificus in vivo. Infect Immun. 2015; 83(8): 3126-36. PMID: 26015477
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Tyagi, R.; Rosa, B.; Lewis, W.G.; Mitreva, M. Pan-phylum Comparison of Nematode Metabolic Potential PLoS Neglected Tropical Diseases. 2015; 9(5):e0003788 PMID: 26000881
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Kline, K.A.; Schwartz, D.J.; Lewis, W.G.; Hultgren, S.J.; Lewis, A.L. 2011. Immune Activation and Suppression by Group B Streptococcus in a Murine Model of Urinary Tract Infection. Infect Immun. 79(9):3588-95. PMID: 21690238
Kinase Inhibitors as Chemical Probes for Biological Processes.
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Chemical probes for biological processes mediated by serine-threonine kinases.
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Cellular and organismal circadian rhythms are controlled by AKT-GSK3b and casein kinases of human and zebrafish, changing the circadian day by many hours.
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Developed cellular kinase inhibitor selectivity panel of phosphorylation assays.
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Biochemical assay development using enzyme and inhibitor conjugates.
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Hirota, T.; Lewis, W.G.; Liu, A.; Lee J.W.; Schultz, P.G.; Kay, S.A. A chemical biology approach reveals period shortening of the mammalian circadian clock by specific inhibition of GSK-3beta. Proc. Nat. Acad. Sci. U S A. 2008. 105(52): 20746-51. PMID: 19104043.
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Hirota, T.; Lee, J.W.; Lewis, W.G.; Zhang,E.E.; Breton,G.; Liu, X.; Garcia, M.; Peters, E.C.; Etchegaray, J-P.; Traver, D.; Schultz, P.G.; Kay, S.A. High-Throughput chemical screen identifies a novel potent modulator of cellular circadian rhythms and reveals CKIα as a clock regulatory kinase. PLoS Biology 2010. 8(12): e1000559. PMID: 21179498
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Liu, A.C.; Lewis, W.G.; Kay, S.A. Mammalian circadian signaling networks and therapeutic targets. Nat. Chem. Biol. 2007. 3: 630-639. (Review) PMID: 17876320
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Wodicke, L.; Lewis, W.G.; Treiber, D.
Cellular Assay Employing Detectable Protein
US2012045769 (A1)
EP 2,421,989
WO 2,010,124,157
(Assignee: DiscoveRx, formerly Ambit Biosciences)