2021

 

Gilbert NM, Choi B, Du J, Collins C, Lewis A, Putonti C, and Wolfe AJ. A mouse model displays host and bacterial strain differences in Aerococcus urinae urinary tract infection. Biology Open (accepted)

 

Agarwal K, Lewis AL. 2021 Vaginal sialoglycan foraging by Gardnerella vaginalis: Mucus barriers as a meal for unwelcome guests? Glycobiology. Apr 5:cwab024. doi: 10.1093/glycob/cwab024. Online ahead of print.

 

The structure and diversity of strain-level variation in vaginal bacteria. Tortelli BA, Lewis AL, Fay JC. Microb Genom. 2021 Mar;7(3). doi: 10.1099/mgen.0.000543. Epub 2021

2020

Gilbert NM, Foster L, Cao B, Yin Y, Mysorekar IU, Lewis AL. Gardnerella vaginalis promotes group B Streptococcus vaginal colonization, enabling ascending uteroplacental infection in pregnant mice. Amer. J. Obstet. Gynecol. November 25, 2020 DOI: https://doi.org/10.1016/j.ajog.2020.11.032 

 

Agarwal KA, Robinson LS, Aggarwal S, Lynne Foster LR, Hernandez-Leyva A, Lin H, Tortelli BA, O’Brien VP, Miller L, Kau AL, Reno H, Gilbert NM, Lewis WG, Lewis AL. Glycan cross-feeding supports mutualism between Fusobacterium and the vaginal microbiota.  PLoS Biology, 2020, Aug 25th  https://doi.org/10.1371/journal.pbio.3000788  BioRxIV 463349; doi: https://doi.org/10.1101/463349

Hilt EE, Putonti C, Thomas-White K, Lewis AL, Visick KL, Gilbert NM, Wolfe AJ. Aerococcus urinae Isolated from Women with Lower Urinary Tract Symptoms: In Vitro Aggregation and Genome Analysis. J Bacteriol. 2020 Jun 9;202(13). doi: 10.1128/JB.00170-20. Print 2020 Jun 9. PubMed PMID: 32284319; PubMed Central PMCID: PMC7283593.

Recurrent Escherichia coli Urinary Tract Infection Triggered by Gardnerella vaginalis Bladder Exposure in Mice.

O'Brien VP, Joens MS, Lewis AL, Gilbert NM.J Vis Exp. 2020 Dec 4;(166):10.3791/61967. doi: 10.3791/61967.PMID: 33346201

 

Morrill S, Gilbert NM, Lewis AL. Gardnerella vaginalis as a Cause of Bacterial Vaginosis: Appraisal of the Evidence From in vivo Models. Front Cell Infect Microbiol. 2020;10:168. doi: 10.3389/fcimb.2020.00168. eCollection 2020. Review. PubMed PMID: 32391287; PubMed Central PMCID: PMC7193744.

 

Lewis AL, Laurent LC. USPSTF 2020 Recommendations on Screening for Asymptomatic Bacterial Vaginosis in Pregnancy. JAMA. 2020 Apr 7;323(13):1253-1255. doi: 10.1001/jama.2019.22311. PubMed PMID: 32259215

 

Lewis AL, Gilbert NM. Roles of the vagina and the vaginal microbiota in urinary tract infection: evidence from clinical correlations and experimental models. GMS Infect Dis. 2020;8:Doc02. doi: 10.3205/id000046. eCollection 2020. Review. PubMed PMID: 32373427; PubMed Central PMCID: PMC7186798. 

Tortelli BA, Lewis WG, Allsworth JE, Member-Meneh N, Foster LR, Reno HE, Peipert JF, Fay JC, Lewis AL. Associations between the vaginal microbiome and Candida colonization in women of reproductive age. Am J Obstet Gynecol. 2020 May;222(5):471.e1-471.e9. doi: 10.1016/j.ajog.2019.10.008. Epub 2019 Oct 22. PubMed PMID: 31654610; PubMed Central PMCID: PMC7236091.

2018-2019

 

O'Brien VP, Gilbert NM, Lebratti T, Agarwal K, Foster L, Shin H, Lewis AL. Low-dose inoculation of Escherichia coli achieves robust vaginal colonization and results in ascending infection accompanied by severe uterine inflammation in mice. PLoS One. 2019;14(7):e0219941. doi: 10.1371/journal.pone.0219941. eCollection 2019. PubMed PMID: 31329630; PubMed Central PMCID: PMC6645506.

 

Gilbert NM, Lewis WG, Li G, Sojka DK, Lubin JB, Lewis AL. Gardnerella vaginalis and Prevotella bivia Trigger Distinct and Overlapping Phenotypes in a Mouse Model of Bacterial Vaginosis. J Infect Dis. 2019 Aug 30;220(7):1099-1108. doi: 10.1093/infdis/jiy704. PubMed PMID: 30715405; PubMed Central PMCID: PMC6736442.

 

Brookheart RT, Lewis WG, Peipert JF, Lewis AL, Allsworth JE. Association between obesity and bacterial vaginosis as assessed by Nugent score. Am J Obstet Gynecol. 2019 May;220(5):476.e1-476.e11. doi: 10.1016/j.ajog.2019.01.229. Epub 2019 Jan 29. PubMed PMID: 30707966; PubMed Central PMCID: PMC7232937.

 

Robinson LS, Schwebke J, Lewis WG, Lewis AL. Identification and characterization of NanH2 and NanH3, enzymes responsible for sialidase activity in the vaginal bacterium Gardnerella vaginalis. J Biol Chem. 2019 Apr 5;294(14):5230-5245. doi: 10.1074/jbc.RA118.006221. Epub 2019 Feb 5. PubMed PMID: 30723162; PubMed Central PMCID: PMC6462536.

 

Gilbert NM, Lewis AL. Covert pathogenesis: Transient exposures to microbes as triggers of disease. PLoS Pathog. 2019 Mar;15(3):e1007586. doi: 10.1371/journal.ppat.1007586. eCollection 2019 Mar. PubMed PMID: 30921443; PubMed Central PMCID: PMC6438445.

 

McDonald ND, DeMeester KE, Lewis AL, Grimes CL, Boyd EF. Structural and functional characterization of a modified legionaminic acid involved in glycosylation of a bacterial lipopolysaccharide. J Biol Chem. 2018 Dec 7;293(49):19113-19126. doi: 10.1074/jbc.RA118.004966. Epub 2018 Oct 12. PubMed PMID: 30315110; PubMed Central PMCID: PMC6295735.

2017

Lin AE, Autran CA, Szyszka A, Escajadillo T, Huang M, Godula K, Prudden AR, Boons GJ, Lewis AL, Doran KS, Nizet V, Bode L. Human milk oligosaccharides inhibit growth of group B Streptococcus. J Biol Chem. 2017 Jul 7;292(27):11243-11249. doi: 10.1074/jbc.M117.789974. Epub 2017 Apr 17. PubMed PMID: 28416607; PubMed Central PMCID: PMC5500792.

 

Gilbert NM, O'Brien VP, Lewis AL. Transient Microbiota Exposures activate dormant Escherichia coli infection in the Bladder and Drive Severe Outcomes of Recurrent Disease. PLoS Pathog. 2017 Mar 30;13(3):e1006238. doi: 10.1371/journal.ppat.1006238. eCollection 2017 Mar. PMID:28358889

 

Robinson LS, Lewis WG, LewisAL. The Sialate O-acetylesterase from Gut Bacteroidetes Species enables Sialidase-mediated Cross Species Foraging of 9-O-acetylated Sialoglycans.  J. Biol. Chem. 2017 Jul 14;292(28): 11861-11872. doi: 10.1074/jbcM116.769232 PMID: 28526748.

 

Amegashie CP, Gilbert NM, Peipert JF, Allsworth JE, Lewis WG, Lewis AL. Relationship between Nugent Score and Vaginal Epithelial Exfoliation.  PLoS One. 2017 May 31;12(5):e0177797. doi: 10.1371/journal.pone.0177797. PMID28562623

 

2016

 

Kline, KA; Lewis, AL. Gram-positive Uropathgens, Polymicrobial Urinary Tract Infection, and the Emerging Microbiota of the Urinary Tract.  Microbiology Spectrum. 2016, March 11; 4(2)

 

Yamaguchi, M; Hirose, Y, Nakata, M; Uchiyama, S; Yamaguchi, Y; Goto, K; Sumitomo, T; Lewis, AL; Kawabata, S; Nizet, V. Evolutionary Inactivation of a Sialidase in Group B Streptococcus. Sci Rep. 2016 Jun 29;6:28852. doi: 10.1038/srep28852. PMID 27352769

 

 Lewis, WG and Lewis, AL. A new Catalog of Microbiological Tools for Women's Infectious Disease Research. Genome Announc. 2016 Sep 29;4(5). pii: e00890-16. doi: 10.1128/genomeA.00890-16. PMID 2768833 

 

O'Brien VP, Hannan TJ, Yu L, Livny J, Roberson ED, Schwartz DJ, Souza S, Mendelsohn CL, Colonna M, Lewis AL, Hultgren SJ. A Mucosal Imprint left by prior E. coli Infection Sensitizes to Recurrent Disease.  Nat Microbiol. 2016 Oct 31;2:16196. doi: 10.1038/nmicrobiol.2016.196. PMID: 27798558

 

Lewis, AL; Robinson, LS; Agarwal, K; and Lewis, WG. Discovery and Characterization of de novo Sialic acid Biosynthesis in the phylum Fusobacterium Glycobiology. 2016 2016 Oct;26(10):1107-1119. doi: 10.1093/glycob/cww068. PMID: 27613803

 

Robinson, LR; Perry, JC; Lek, S; Wollam, A; Sodergren, E; Weinstock, G; Lewis, AL; Lewis, WG.  Draft Genome Sequences of 15 Gardnerella vaginalis strains Isolated from the Vaginas of Women with and without Bacterial Vaginosis.  Genome Announc. 2016 Sep 29;4(5). pii: e00879-16. doi: 10.1128/genomeA.00879-16. PMID 27688326

 

Lewis, AL; Deitzler, G; Ruiz, M; Weimer, C; Park, S; Robinson, L; Wollam, A; Hallsworth-Pepin, K; Mitreva, M; Lewis; WG. Draft Genome Sequences of 11 human vaginal isolates of Actinobacteria. Genome Announc. 2016 Sep 29;4(5). pii: e00887-16. doi: 10.1128/genomeA.00887-16. PMID 27688328

 

Deitzler, G; Ruiz, M; Weimer, C; Park, S; Robinson, L; Wollam, A; Hallsworth-Pepin, K; Mitreva, M; Lewis, AL; Lewis, WG. Draft Genome Sequences of 14 Firmicutes isolated from the Human Vagina. Genome Announc. 2016 Sep 29;4(5). pii: e00888-16. doi: 10.1128/genomeA.00888-16. PMID 27688329

 

Deitzler, G; Ruiz, M; Lu, W; Robinson, L; Weimer, C; Park, S; Wollam, A; Hallsworth-Pepin, K; Mitreva, M. Lewis, WG; Lewis, AL; Draft Genome Sequences of Seven Gram-negative Vaginal Bacteria. Genome Announc. 2016 Sep 29;4(5). pii: e00889-16. doi: 10.1128/genomeA.00889-16. PMID 2768833

 

Weimer, C; Deitzler, G; Ruiz, M; Robinson, L; Park, S; Hallsworth-Pepin, K; Mitreva, M; Lewis, W; Lewis, AL. Draft Genome Sequences for 12 Bacterial Isolates Obtained from the Urine of Pregnant Women. Genome Announc. 2016 Sep 29;4(5). pii: e00882-16. doi: 10.1128/genomeA.00882-16. PMID 27688327
 

2014-2015

 

Kline, K.A.; Schwartz, D.J.; Gilbert, NM; Lewis, A.L. Impact of Host Age and Parity on Susceptibility to Severe Urinary Tract Infection in a Murine Model. PLoS One. 2014 May 16;9(5):e97798. PMID: 24835885

 

Lubin, J.B.; Lewis, W.G.; Gilbert, N.M.; Almagro-Moreno, S.; Boyd, E.F.; Lewis, A.L Host-like carbohydrates promote bloodstream survival of Vibrio vulnificus in vivo. Infect Immun. 2015 Aug;83(8):3126-36. Epub 2015 May 26.PMID: 2601547

2013

Lewis, WG; Robinson, LS; Gilbert, NM; Lewis, AL. Degradation, Foraging, and Depletion of Mucus Sialoglycans by the Vaginal-Adapted Actinobacterium Gardnerella vaginalis. J Biol Chem. 2013 Apr 26;288(17):12067-79. PMID: 23479734

Faculty of 1000 Recommended, Highlighted in Town and Style magazine and Fit Pregnancy Magazine              

Gilbert, NM; Lewis, WG; Robinson, LS;  Lewis, AL. Clinical Features of Bacterial Vaginosis in a Murine Model of Vaginal Infection with Gardnerella vaginalis. PLoS One. 2013;8(3):e59539. doi: 10.1371/journal.pone.0059539. Epub 2013 Mar 19. PMID: 23527214

Highlighted in Town and Style magazine and Fit Pregnancy Magazine

 

Gilbert, NM; O’Brien, V; Hultgren, S; Macones, GA; Lewis, WG; Lewis, AL. Urinary Tract Infection as a Preventable Cause of Pregnancy Complications: Opportunities, Challenges, and a Global Call to Action.   Global Adv. Health Med. 2013;2(5):59-69. PMID: 24416696

 

2012

Lewis, WG; Robinson, LS; Perry, JC; Bick, JL; Peipert, JF; Allsworth, JE; Lewis, AL. 2012. Hydrolysis of Secreted Immunoglobulin A (IgA) in ex Vivo and Biochemical Models of Bacterial Vaginosis. J Biol Chem. 287(3): 2079-89. PMID: 22134918

Highlighted in the ASBMB Blog “Wild-types”

 

Ricaldi,JN; Matthias, MA; Vinetz, JM; Lewis, AL. 2012 Expression of Sialic Acids and other Nonulosonic Acids in Leptospira. BMC Microbiol. 12 (1):161. PMID: 22853805

 

Kline, K.A.; Schwartz, D.J.; Gilbert, NM; Hultgren, S.J.; Lewis, A.L. Immune Modulation by Group B Streptococcus  Influences Host Susceptibility to Urinary Tract Infection by Uropathogenic E. coli. Infect. Immun. 2012 Dec;80(12):4186-94. PMID 22988014

 

Lewis AL, Lewis WG. 2012. Host Sialoglycans and Bacterial Sialidases: A Mucosal Perspective. Cell Microbiol. 2012 14(8):1174-82. PMID: 22519819

 

2010-2011

 

Weiman S, Uchiyama S, Lin FY, Chaffin D, Varki A, Nizet V, Lewis AL. 2010. O-Acetylation of Sialic Acid on Group B Streptococcus Inhibits Neutrophil Suppression and Virulence. Biochem J. 428(2):163-8.

 

Lewis, A.L.; Lubin, J.B.; Argade, S.; Naidu, N.; Choudhury, B.; and Boyd, E.F. 2011. Genomic and Metabolic Profiling of Nonulosonic Acids in Vibrionaceae reveal Biochemical Phenotypes of Allelic Divergence in V. vulnificus Appl. Environ. Microbiol. Aug;77(16):5782-93. PMID: 21724895

 

Kline, K.A.; Schwartz, D.J.; Lewis, W.G.; Hultgren, S.J.; Lewis, A.L. 2011. Immune Activation and Suppression by Group B Streptococcus in a Murine Model of Urinary Tract Infection. Infect Immun. Sep;79(9):3588-95. PMID: 21690238

 

2009

 

Carlin, A.; Uchiyama, S.; Chang, Y.; Lewis, A.L.; Nizet, V.; Varki, A. 2009. Molecular Mimicry of Host Sialylated Glycans Allows a Bacterial Pathogen to Engage Neutrophil Siglec-9 and Dampen the Innate Immune Response. Blood. 113(14):3333-6.

 

Pannaraj PS, Edwards MS, Ewing KT, Lewis AL, Rench MA, Baker CJ. 2009. Group B streptococcal conjugate vaccines elicit functional antibodies independent of strain O-acetylation. Vaccine.16;27 (33):4452-6.

 

Weiman S, Dahesh S, Carlin AF, Varki A, Nizet V, Lewis AL. 2009. Genetic and Biochemical Modulation of Sialic Acid O-Acetylation on Group B Streptococcus: Phenotypic and Functional Impact. Glycobiology. (11):1204-13.

 

Lewis, A.L.; Varki, A. Evolutionary Considerations in Studying the Sialome: Sialic Acids and the Host-Pathogen Interface. Bioinformatics for Glycobiology and Glycomics: An Introduction.  pgs 69-88. Wiley & Sons Ltd. 2009.

 

Lewis, A.L.; Desa, N.; Hansen, E.E.; Knirel, Y.; Gordon, J.; Gagneux, P.; Nizet, V.; Varki, A. 2009. Innovations in Host and Microbial Sialic Acid Biosynthesis Revealed by Phylogenomic Prediction of Nonulosonic Acid Structure. Proc. Natl. Acad. Sci. U S A. 11;106 (32):13552-7.

 

2004-2007

 

Lewis, A.L.; Cao, H.; Patel, S.K.; Diaz, S.; Ryan, W.; Carlin, A.F.; Thon, V.; Lewis, W.G.; Varki, A.; Chen, X.; Nizet, V. 2007. NeuA sialic acid O-acetylesterase activity modulates O-acetylation of capsular polysaccharide in Group B Streptococcus. J. Biol. Chem. 282 (38):27562-71.

 

Carlin, A.F.; Lewis, A.L.; Varki, A.; Nizet, V. 2007. Group B streptococcal capsular sialic acids interact with siglecs (immunoglobulin-like lectins) on human leukocytes. J Bacteriol. 189(4):1231-7.

 

Lewis, A.L.; Hensler, M.; Nizet, V; Varki, A. 2006. The group B streptococcal sialic acid O-acetyltransferase is encoded by neuD, a conserved component of bacterial sialic acid biosynthetic gene clusters. J Biol Chem.  281(16):11186.

 

Lewis, A. L.; Altheide, T.K.; Varki, A.; Arden, K.; Varki, N.M. 2005. Combining Parenting and a Science Career. Science 307(5716): 1720.

 

Hayakawa, T.; Angata,T.; Lewis, A.L.; Mikkelsen, T.; Varki, N.; Varki, A. 2005. A human-specific gene in microglia. Science 309 (5741):1693.

 

Lewis, A.L.; Nizet, V.; Varki. A.  2004. Discovery and Characterization of Sialic Acid O-Acetylation in Group B Streptococcus. Proc. Nat. Acad. Sci. U.S.A. 101(30): 11123-11128

 

Doolittle, R.F.; York, A.L. 2002. Bacterial Actins? An Evolutionary Perspective. BioEssays 24: 293-296.

(AL Lewis, maiden name York)